Fig. 5

CBX7 regulates stem cell characteristics (GCSC phenotype) of gastric cancer cells by constitutive activation of AKT. a Western blot analysis was used to detect pAKT, total AKT, CBX7, and β-actin expression in control and SGC-7901 cells expressing pSRα-mAkt or CBX7i. β-actin served as an internal control. b Control and SGC-7901 cells expressing pSRα-mAkt and/or CBX7i were subjected to colony formation assay. c Control and SGC-7901 cells expressing pSRα-mAkt and/or CBX7i were subjected to migration and invasion assays. d Control and SGC-7901 cells expressing pSRα-mAkt and/or CBX7i were analyzed using spheroid formation assay. e The chemoresistance of control and SGC-7901 cells expressing pSRα-mAkt and/or CBX7i were determined. EPI epirubicin. ***P < 0.001