Fig. 2

In vitro and in vivo functions of NLRX1 in HCC. a Expression of NLRX1 in 6 HCC cell lines determined by RT-PCR and WB. b Validation of NLRX1 modulation by RT-PCR and WB. c Immunofluorescent staining for NLRX1 in Huh7 cells. d Effect of NLRX1 on cell apoptosis as assessed by flow cytometry. e Evaluation of the effects of NLRX1 on HCC invasion by Transwell assays. f Evaluation of the effects of NLRX1 on tumor proliferation in vitro by CCK-8 assays. g Effect of NLRX1 on tumor proliferation in vivo. All in vitro experiments were conducted triplicate; one asterisk and three asterisks indicate P < 0.05 and P < 0.001, respectively