Fig. 5

Knockdown of CAMK1 or CAMK4 blocks xenograft of human leukemia cells. a Survival curve of NSG mice transplanted with MV4-11 cells (1 × 10⁶ cells) infected with virus designed to express GFP and either scrambled shRNA, Camk1 shRNA, or Camk4 shRNA. GFP⁺ cells were collected and transplanted into NSG mice 1 day post-infection (n = 10 mice). b Percentages of GFP⁺ cells in BM, spleen, liver, and PB at 28 days after transplantation. c Representative flow cytometry plots indicating decreased spleen engraftment of MV4-11 cells treated with shRNA targeting Camk1 or Camk4. Staining with anti-human CD45 antibodies confirmed engraftment was from transplanted human leukemia cells. The percentages of hCD45⁺ population are indicated. d Comparison of the sizes of spleens of the mice transplanted with control MV4-11 leukemia cells or cells expressing shRNA targeting Camk1 or Camk4 (n = 5 mice); *p < 0.05