Fig. 6

ASA suppresses HBXIP/HOXB13 axis by reducing HBXIP expression. a qRT-PCR assay of HBXIP, HOXB13, and IL-6 in BT474 cells treated with the displayed doses of ASA for 24 h. b Immunoblotting analysis of HBXIP and HOXB13 in BT474 cells treated with 2.5 mM ASA for the indicated time points (lower panel). The upper panel is the quantification of the intensity relative to β-actin. c Immunoblotting analysis of HBXIP and HOXB13 in BT474 cells treated with different concentrations of ASA for 24 h (lower panel). The upper panel is the quantification of the intensity relative to β-actin. d ELISA of IL-6 secretion in MCF-7-HBXIP cells treated with the indicated doses of ASA for 24 h. e qRT-PCR assay of HBXIP, IL-6, STAT3, and ER-α in BT474 cells treated with DMSO or ASA (2.5 mM) for 24 h after being transiently transfected with pCMV or pCMV-HBXIP. f Immunoblotting analysis of HBXIP, HOXB13, STAT3, and ER-α in BT474 cells treated with DMSO or ASA (2.5 mM) for 24 h after being transiently transfected with pCMV or pCMV-HBXIP. g The quantification of the intensity relative to β-actin in f. All experiments were repeated at least three times. Error bars represent ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 by two-tailed Student’s t test