Fig. 7
ASA-inhibited HBXIP/HOXB13 axis contributes to the reversal of TAM resistance. Growth curve (a) and imaging (b) of the xenograft tumors derived from BT474 cells with β-estradiol supplementation. After the tumors reached an approximate volume of 150 mm3, the mice were randomized into four treatment groups and were treated daily with the gavage administration of physiological saline (Veh), ASA (suspended in physiological saline, 75 mg/kg), TAM (suspended in physiological saline, 5 mg/kg), or a combination of ASA and TAM (TAM + ASA). c Weights of the xenograft tumors derived from BT474 cells shown in a. d Ki67 staining by IHC assay and the statistics of the Ki67-positive cells of the xenograft tumors derived from BT474 cells shown in a. Scale bar, 100 μm. e qRT-RCR assay of IL-6 expression in the xenograft tumors derived from BT474 cells shown in a. f Immunoblotting analysis of HBXIP, HOXB13, and ER-α in the xenograft tumors derived from BT474 cells shown in a. Error bars represent ± SD. **P < 0.01 and ***P < 0.001 by two-tailed Student’s t test