Fig. 2

B3GALNT2 confers no effects on HCC cells in vitro whereas it promotes tumor growth in vivo. a, b The mRNA and protein level of B3GALNT2 in L02 and seven hepatocellular carcinoma cell lines was determined by qPCR and Western blot. c, d Knockdown and overexpression efficiency of B3GALNT2 in Huh7 and 7402 cell lines was determined by qPCR and Western blot. e CCK-8 analysis of Huh7-shB3GALNT2 and 7402-B3GALNT2 cells and their control cells. f In vivo effect of B3GALNT2 in subcutaneous xenograft model at 4th week after inoculation (n = 6 in each group). Representative photos of tumor-bearing mice are shown on the left, and statistical data is shown on the right. g In vivo effect of B3GALNT2 in an orthotopic transplantation model 4 weeks after tumor transplantation (n = 6 in each group). Representative images of tumor-bearing mice are shown on the left, and statistical data is shown on the right. h Overall survival of orthotopic transplanted mice. *p < 0.05, n.s. not significant