Fig. 5
miR-519a enhanced the antitumor efficacy of TMZ in vivo. The dissected tumors were collected at the end of drug administration (a), and tumor weight was measured (b). The tumor volume was calculated as 4/3 × 3.14 × radius (mm)3 (c). Immunohistochemical analysis (d) and Western blot analysis (e) of phospho-STAT3, LC3B, Bcl-2, Bax, and cleaved caspase-3 levels in xenograft tumors. Xenograft tumors were subjected to TEM. AV (autophagic vacuoles) = autophagosomes and lysosomes (f, g). Coronal T2-weighted MRI of tumors acquired from patient-derived GBM cells (G131212/TMZ) from one animal in each treatment group (red arrow) in the brain samples on day 24 after treatment (h). The survival of mice with orthotopic tumors was measured by Kaplan-Meier survival curves (i). *p < 0.05, **p < 0.01