Fig. 1

WEE1 overexpression in ALL samples. a WEE1 transcript levels in samples isolated from adult ALL (n = 58) and in MNCs (n = 7) from peripheral blood of healthy donors. One-way ANOVA test was performed to confirm the statistical significance of the differences. Results are expressed as Log10 2exp[−(ΔΔCt). b WEE1 transcript levels in samples isolated from adult BCR-ABL1-positive (Ph+) ALL at diagnosis (n = 17), adult BCR-ABL1-negative (Ph−) ALL at diagnosis (n = 27), adult BCR-ABL1-positive ALL at relapse (unpaired, n = 8), adult BCR-ABL1-negative ALL at relapse (unpaired, n = 6), and in MNCs (n = 7) from the peripheral blood samples of healthy donors. Results are expressed as Log10 2exp[−(ΔΔCt). c Immunohistochemistry analysis of a reactive bone marrow sample; WEE1 is positive at moderate to strong intensity in morphologically typical myeloid precursors (× 20). d Immunohistochemistry analysis of leukemic blasts scattered in the interstitium and positive for WEE1 (× 20). e Leukemic blasts are widely positive for the B cell marker CD79a (red) while those positive for WEE1 (brown) are much fewer, as shown by the few scattered double-stained blasts (× 20)