Fig. 2

PIK3CA E542K and E545K mutations promote proliferation and glucose metabolism in cervical cancer cell. a Transfection of FLAG-tagged PI3K E542K and E545K in SiHa and MS751 cells. b Detection of proliferation by colony formation in SiHa and MS751 cells with wild-type and mutant PIK3CA (**P < 0.01). c The effect of PIK3CA E542K and E545K mutation on glucose uptake (**P < 0.01). d The effect of PIK3CA E542K and E545K mutation on lactate production (**P < 0.01). e ECAR analysis in SiHa and MS751 cells with wild-type and mutant PIK3CA. f PIK3CA E542K and E545K mutation affected ATP production (**P < 0.01). g Relative mRNA expression of key enzymes of glycolysis in SiHa and MS751 cells with wild-type and mutant PIK3CA (*P < 0.05; **P < 0.01). h The effect of PIK3CA E542K and E545K mutation on the expression level of key glycolytic enzyme