Fig. 8

Six2-mediated regulation of ceRNET_CC renders breast cancer cell resistance to adriamycin treatment by promoting cell stemness. a CYP4Z2P, CYP4Z1, and six2 expression were detected in MCF-7 and MCF-7-Adr cells by qRT-PCR. b MCF-7-Adr cells were transfected with si-CYP4Z1, si-CYP4Z2P, or si-six2 plus Z1-UTR, or Z2P-UTR and followed by detecting the expression of p-Akt/p-ERK1/2, P-gp, and stemness markers (ALDH1 and OCT3/4). c Phase contrast images of mammospheres formed by cells depicted in b, and spheres were quantified. The data are presented as the means ± SDs, n = 3, **P < 0.001 vs. MCF-7. d Representative FACS profile of cells described in b with CD24⁻ and CD44⁺ markers. e, f Images (e) and number (f) of tumors harvested when cells described in b were seeded