Fig. 3

SPAG5 expression promotes cell cycle progression and inhibits cell apoptosis of TNBC. a Flow cytometry indicates that overexpression of SPAG5 promotes S to G2 transition in MDA-MB-231 and MDA-MB-468 cells, while knockdown of SPAG5 suppress S to G2 transition in Hs-578t and BT549 cells. b Flow cytometry indicates that overexpression of SPAG5 inhibit cell apoptosis in MDA-MB-231 and MDA-MB-468 cells, while knockdown of SPAG5 increase cell apoptosis in Hs-578t and BT549 cells. c Western blot of SPAG5, P21, P27, Cyclin A2, Cyclin B1, and CDC20 in MDA-MB-231 and MDA-MB-468 cells treated with SPAG5 overexpression and Hs-578t and BT549 cells treated with SPAG5 siRNAs. d Tumors formed by SPAG5 overexpression MDA-MB-231 cells were larger than those formed by cells transduced with the vector control. All *p<0.05, **p<0.01, ***p<0.001, n.s. not significant