Skip to main content
Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: SPAG5 upregulation contributes to enhanced c-MYC transcriptional activity via interaction with c-MYC binding protein in triple-negative breast cancer

Fig. 6

SPAG5 upregulates transcription of c-MYC target genes by regulating c-MYC transcriptional activity. a Cell cycle-related genes and ATR BRCA pathway related genes showed differential mRNA expression in SPAG5 overexpression cells compared with that in vector cells in RNA-sequencing analysis. b RT-qPCR of CDC20, CDC25C, RAD51, BRCA1, and BRCA2 in BT549 cells treated with SPAG5 siRNAs. c Correlation of SPAG5 and CDC20, CDC25C, RAD51, BRCA1, and BRCA2 mRNA levels in GSE76250 dataset. d CHIP-qPCR showed SPAG5 knockdown in BT549 cells significantly reduced c-MYC’s occupancies on the promoters of CDC20, CDC25C, RAD51, BRCA1, and BRCA2. All *p<0.05, **p<0.01, ***p<0.001, n.s. not significant

Back to article page

Journal of Hematology & Oncology

ISSN: 1756-8722

Contact us