Fig. 8

c-MYC is partially involved in SPAG5 regulated breast cancer cell growth. CCK-8 (a) and colony-forming (b) assays showed that knockdown of c-MYC partially attenuated the enhanced cell proliferation induced by overexpression of SPAG5 in MDA-MB-231 and MDA-MB-468 cells. c Flow cytometry indicates that knockdown of c-MYC partially reverse the increased S to G2 transition induced by overexpression of SPAG5 in MDA-MB-231 and MDA-MB-468 cells. d Flow cytometry indicates that knockdown of c-MYC partially reverse the reduced cell apoptosis induced by overexpression of SPAG5 in MDA-MB-231 and MDA-MB-468 cells. e CCK8 assays showed IC50 values for olaparib in SPAG5 overexpression MDA-MB-231 and MDA-MB-468 cells transfected with c-MYC siRNAs. f MDA-MB-231and MDA-MB-468 cells stably expressing SPAG5 were infected with siRNAs targeting c-MYC. Western blot was used to detect c-MYC, CDC20, Cyclin A2, Cyclin B1, RAD51, BRCA1 and BRCA2 protein expression level. g Model showing the effect of SPAG5/MYCBP/c-MYC signaling on breast tumor growth. All *p<0.05, **p<0.01, ***p<0.001, n.s. not significant