Fig. 2

miR-195-5p inhibits HCT116 and DLD-1 cell proliferation, migration, and invasion in vitro. a, b Representative photomicrographs and quantifications of clone formation assay in DLD1 and HCT116 cells after transfection with miR-195-5p mimic, miR-195-5p mimic NC, miR-195-5p inhibitor, or miR-195-5p inhibitor NC for 48 h. c, d Representative photomicrographs and quantifications of EdU immunofluorescence staining assay in DLD1 and HCT116 cells. Bar = 50 μm. e, f Photomicrographs and quantifications of wound healing assay. Bar = 100 μm. g Transwell migration assays of DLD1 and HCT116 cells carrying different miRNAs. Bar = 100 μm. h Total number of cells in five fields was counted manually. i Transwell invasion assays of DLD1 and HCT116 cells. Bar = 100 μm. j Total number of cells in five fields was counted manually. Mean ± SD are shown. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001