Fig. 3

HLA-DR^−/lowCD33⁺CD16⁻ cells could affect the balance of Th subsets induce Treg generation. a Treg (CD4⁺CD25⁺Foxp3⁺) generation was detected through flow cytometry. CD3⁺ T cells were co-cultured with or without HLA-DR^−/lowCD33⁺ CD16⁻ cells for 4 days. b The percentage of Treg in CD4⁺ T cell, group HLA-DR^−/lowCD33⁺CD16⁻ vs. control, p = 0.0048. c Flow cytometry analysis of Th1 (CD4⁺IFN-γ⁺), Th2 (CD4⁺IL-4⁺), Th17 (CD4⁺IL-17a⁺). CD3⁺ T cells were co-cultured with or without HLA-DR^−/lowCD33⁺CD16⁻ cells sorted from GPBSC in the presence of CD3/CD28 for 4 days. Red for CD4+ cell fraction and blue for CD4-negative part. d Th2/Th1 ratio fold change compared with the control group, p = 0.0159. e Th2/(Th1+Th17) ratio fold change compared with the control group, p = 0.0095