Fig. 6

Dual viruses-loaded MSCs delivering CD3-HAC to metastatic niche. a Frozen sections of the lungs from 231-Luc tumor-bearing mice sacrificed at indicted time points after MSC.Adtrack.LentiR. or MSC.Adtrack.E1A infusion showed the change of gene expression of engineered MSC in vivo. Blue (nuclei). Green (GFP) represented the cells infected by adenovirus. Red (anti-Luc) represented MDA-MB-231 cells. White (DsRed) represented the expression of E1A. Pictures of each channel were displayed in Additional file 1: Fig. S9. b MSC.CD3-HAC.E1A or MSC.Adtrack.E1A were injected intravenously into 231-Luc hosting mice, followed by PBMCs infusion after 2 days. IFN-γ⁺ cells were detected in tumor sites. White arrows indicated the interaction between the IFN-γ⁺ cells and adenovirus-infected tumor cells. Green (GFP) represented the cells infected by adenovirus. Red (Cell Trace™ Far Red) represented PBMCs. White (anti-IFN-γ). Scar bar, 50 μm