Fig. 2From: CD73 promotes hepatocellular carcinoma progression and metastasis via activating PI3K/AKT signaling by inducing Rap1-mediated membrane localization of P110β and predicts poor prognosisBiological functions of CD73 in HCC. a Efficiencies of CD73 knockdown and overexpression were validated by RT-PCR (left) and WB (right) assays. b Evaluation of the influence of CD73 on HCC proliferation by CCK-8. c Evaluations of the influence of CD73 on HCC proliferation by colony formation assays. d Evaluations of the influence of CD73 on cell cycle in HCC cells by flow cytometry. e Evaluations of the influence of CD73 on apoptosis in HCC cells by flow cytometry. f Evaluations of the influence of CD73 on migration and invasion activities of HCC cells by Transwell assays. g Evaluations of the influence of CD73 on migration activities of HCC cells by wound healing assays. h Establishment of the orthotopic xenograft model with NOG mice. Tumor volumes are shown in the right panel. i Incidences of intrahepatic (left) or lung (right) metastasis in indicated orthotopic xenograft model groups. All in vitro experiments were performed in triplicate; asterisk indicated P < 0.050, and t tests were usedBack to article page