Fig. 9
From: High-affinity peptide ligand LXY30 for targeting α3β1 integrin in non-small cell lung cancer
LXY30 specifically targets subcutaneous PDX xenograft tumors. A lung squamous cell (LUSC) PDX model was generated by engrafting biopsy specimen into NSG mice as described in the “Materials and methods” section. Optical images by white light (WL) and in vivo fluorescence images (a) were taken 6 h after the injection of streptavidin-Cy5.5 (SA-Cy5.5) either alone or together with biotinylated LXY30 (LXY30-biotin) into NSG mice bearing subcutaneous LUSC PDX xenografts. Major organs from the NSG mice were subjected to ex vivo imaging immediately after the mice were sacrificed (b). The fluorescence signals of SA-Cy5.5 and LXY30-Biotin-SA-Cy5.5 were further quantitated for subcutaneous LUSC PDX tumors (c). Representative images of LUSC PDX tumor sections were stained by fluorescent (Alexa594)-labeled anti-α3 integrin antibody, H&E, integrin α3, and integrin β1 immunohistochemistry stains (d). PDX, patient-derived xenograft; LUSC, lung squamous carcinoma; WL, white light