Fig. 6

LAT2 and OLR1 are the direct targets of let-7. a Target scan predicted the let-7 binding sites of 3′-UTR of LAT2. Mutation was generated on the 3′-UTR of LAT2 sequence for the seed region of let-7. b Target scan predicted the let-7 binding sites of 3′-UTR of OLR1. Mutation was generated on the 3′-UTR of OLR1 sequence for the seed region of let-7. c The luciferase activities of 3′-UTR of LAT2 reporter vector were reduced when co-transfected with let-7 mimics; whereas, let-7 had no effects on luciferase activities of 3′-UTR of LAT2 reporter vector. d The luciferase activities of 3′-UTR of OLR1 reporter vector were reduced when co-transfected with let-7 mimics; whereas, let-7 had no effects on luciferase activities of 3′-UTR of OLR1 reporter vector. e, f Western blot analysis showed that the expression levels of LAT2 and OLR1 were downregulated when let-7 overexpresses in AsPC-1 and MIAPaCa-2 cells. The data are presented as the mean ± SD (Student’s t test; asterisk indicates P < 0.05)