Fig. 2
From: MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells
The detection of MAGE-A1 expression in LUAD cell lines. a, b Detection of MAGE-A1 expression in LUAD cell lines (PC9, H1299, GLC82, A549) by qPCR and western blotting analyses. The human normal bronchial epithelial (HBE) cell line was used as a non-cancerous control cell line. *Significant difference of MAGE-A1 expression in LUAD cell lines compared with HBE cells. p < 0.05. c Immunofluorescence assay revealed that MAGE-A1 could be stained in MAGE-A1-positive PC9 cell but not in MAGE-A1-negative HBE cell. The human melanoma A375 cell line was employed as a positive control and MAGE-A1-positive staining could also be observed in A375 cell line. Strong staining of MAGE-A1 was mainly localized in the cytomembrane while relatively weak staining of MAGE-A1 was observed in cytoplasm of cancer cells. Green, MAGE-A1 staining; red, DSG-2 staining; blue, nuclear staining