Fig. 3
From: Simultaneous targeting of XPO1 and BCL2 as an effective treatment strategy for double-hit lymphoma
KPT8602 synergizes with ABT199 in vitro to kill DHL tumor cells. a Cell viability upon treatment with KPT8602 (100 nM) and ABT199 (40 nM for DHL4/DHL6, and 20 nM for Toledo) for 48 h (two-tailed test: ** indicates P < 0.0001; * indicates P < 0.001). b–d DHL cells were treated with 80 nM ABT199, 100 nM KPT8602, or both for 8 or 24 h. Western blot analysis of protein lysates for MYC, cleaved PARP (C-PARP), caspase 3 (Casp3), cleaved caspase 3 (C-Casp3), BCL2, and β-tubulin (Tub). e Flow cytometric analysis of apoptosis induction in DHL4 treated with 100 nM ABT199 and 50 nM KPT8602 for 48 h. Cells were stained with annexin-V and 7-AAD