Fig. 7

Effect of anti-CD47 and anti-PD-L1 treatment on tumor-infiltrating immune cells in PDAC mouse models. Panc02 or MPC-83 cells were transplanted to C57BL/6 or KM mice. And the tumor-bearing mice were treated as in Fig. 6 c and d. Cell suspensions were prepared from the peripheral blood, spleen, and pancreatic tumor tissues from tumor-bearing mice and analyzed by flow cytometry and bulk RNA-seq. a–c Quantification of CD8⁺PD-1⁺ T lymphocytes in the Panc02 tumor model in the indicated tissues. d–f Quantification of CD8⁺ PD-1⁺ T lymphocytes in the MPC-83 tumor model in the indicated tissues. g Heatmap from RNA-seq displaying expression of select genes in each tumor-bearing mouse group