Fig. 2

Knockdown of LINC00958 inhibits HCC proliferation, migration, and invasion in vitro. a Levels of LINC00958 from the nuclear and cytoplasmic fractions of HCCLM3 and Focus cells were evaluated using RT-qPCR. GAPDH and U6 were used as positive control for the cytoplasmic and nuclear fraction, respectively. b FISH was performed to determine the subcellular distribution of LINC00958 in HCCLM3 cells. c The expression of LINC00958 was knocked down using three shRNAs in HCCLM3 and Focus cells. d CCK-8 assays were performed to assess the cell proliferation in LINC00958-silenced HCC cells. e Colony formation assays showed the clone numbers in HCC cells with LINC00958 knockdown. f EdU assays were performed to assess the proliferative ability of HCCLM3 and Focus cells with LINC00958 knockdown. g Transwell assays were conducted to examine the effects of LINC00958 knockdown on HCC cell migration and invasion. *P < 0.05, **P < 0.01, ***P < 0.001