Fig. 2

AGK promotes RCC cell proliferation. a Western blotting reveals that AGK was efficiently knocked down or overexpressed in corresponding cells. GAPDH was used as a loading control. b MTT assay and c colony formation assay showing the proliferation of the indicated RCC cells. Data are presented as the mean ± SD of three independent experiments. d, e Representative micrographs (left panel) of EDU incorporation detected in the indicated RCC cells. f, g Flow cytometric analysis (right panel) of the indicated RCC cells. The error bars represent the standard deviation of the mean (SD) calculated from three experiments performed in parallel. P-values were calculated using an independent Student’s t-test. *P <  0.05; **P <  0.01; ***P <  0.001; ns means non-significant