Fig. 1

Preclinical evaluation of bispecific CD19/CD22 CAR T cells. a Schematic of the bispecific CD19/CD22 CAR structure. b Cytotoxicity of CAR T cells against target cells carrying luciferase reporter gene evaluated by luminescent assay, after co-culturing with tumor cells for 4 h at the indicated E:T ratios, with CAR T cells. c Cytokine production by anti-CD19 CAR-, anti-CD22 CAR-, and bispecific CAR-expressing T cells co-incubated with K562, K562-CD19, K562-CD22, and K562-CD19CD22 cell lines. Bars represent mean + SD of replicate samples. Data are representative of three independent experiments performed with CAR T cells from three separate donors. A two-tailed, unpaired two-sample t test was used for statistical analysis