Fig. 5

HUMT exerted its function by regulating the FOXK1 expression and downstream signaling. a Western blot analysis of the corresponding signaling in HUMT-KO- and FOXK1-overexpressed MDA-MB-231 cells. b–d Representative graphs and quantification of wound healing assay, Transwell migration, and invasion assay in the MDA-MB-231 and BT549 cells cotransfected with HUMT overexpression vector or empty vector together with si-FOXK1 or scrambled control. e Representative pictures of tube formation assay in HLECs cultured in medium supernatant of the abovementioned cells. f Quantitative analysis of the branch number and total tube length in tube formation assay. g Representative graphs and quantification of Transwell migration assay in HLECs cultured in medium supernatant of the abovementioned cells. h Western blot analysis of the corresponding signaling in MDA-MB-231 and BT549 cotransfected with HUMT overexpression vector or empty vector together with si-FOXK1 or scrambled control. Data were shown as mean ± SD; *P < 0.05; **P < 0.01