Fig. 6

Antineoplastic activity of T22-GFP-H6-Auristatin in the bone marrow, circulating blood, and affected extramedullar sites ex vivo. a Comparison of bioluminescence emission between the T22-GFP-H6-Auristatin-treated (T22-AUR, orange bars), vehicle-treated (VEH, blue bars), and untreated healthy mice (Normal, green bars) groups in the bone marrow, liver, and spleen. b Bioluminescence Images comparing leukemia dissemination in the bone marrow, liver, and spleen of mice treated with the nanoconjugate (T22-AUR) or vehicle (VEH), and untreated healthy mice (Normal). c Comparison of levels of bioluminescence emitted by circulating blood between groups (VEH vs T22-AUR) detected ex vivo at the end of the experiment. d Detection of CD45-positive cells in the peripheral blood by flow cytometry in mice treated with vehicle (VEH, blue) or T22-GFP-H6-Auristatin (T22-AUR, orange). Results are presented as percentage, normalizing the number of CD45-positive cells found in a sample by the mean of CD45-positive cells found in all vehicle-treated mice. e Histogram and dot plot representations of FL3-H fluorescence detection in a mouse blood sample treated with vehicle (VEH, blue) overlapped with another treated with T22-GFP-H6-Auristatin (T22-AUR, orange). M1 and R2 sections indicate CD45-positive cells, whereas R1 section indicates CD45-negative cells. Results in studies a and c are presented as mean ± SE luminescence values in photons per second (total flux [p/s]), and as mean ± SE of the percent of CD45-positive cells in d. U of Mann-Whitney test was used in studies a, c, and d to assess significant differences and are presented in the figures with an * to compare between the VEH and T22-AUR groups, # between the Normal and VEH groups, and ‡ between the Normal and T22-AUR groups. The differences between groups were considered significant when the p value was lower than 0.05. PB, peripheral blood; T22-AUR, T22-GFP-H6-Auristatin group; VEH, vehicle group; SE, standard error