Fig. 5

Pathology analysis of the syngeneic mice injected with saline, p185^Bcr-Abl Cas9, p185^Bcr-Abl KO2.3, and p185^Bcr-Abl KO6.2 cells. a. Liver and spleen weights of mice injected with saline (control), p185^Bcr-Abl Cas9 (p185 Cas9), p185^Bcr-Abl KO2.3 (p185 KO2.3), and p185^Bcr-Abl KO6.2 (p185 KO6.2) cells. b. Spleens from the mice received saline as control or p185 Cas9, p185 KO2.3, and p185 KO6.2 cells, as indicated. c. Histology of livers and spleens from the mice that received saline as control or p185 Cas9, p185 KO2.3, and p185 KO6.2 cells, as indicated. Livers and spleens were collected from moribund mice received p185 Cas9 control cells and the age-matched mice received p185 KO2.3, p185 KO6.2 cells, or saline as control. Collected tissues were fixed in 10% formalin for 24 h and then paraffin-embedded. The sections from embedded tissues were stained with hematoxylin and eosin. Arrows indicate the massively expanded p185 Cas9 control cells in spleen and liver that are morphologically distinguishable from normal tissue cells