Skip to main content
Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Antibody targeting tumor-derived soluble NKG2D ligand sMIC reprograms NK cell homeostatic survival and function and enhances melanoma response to PDL1 blockade therapy

Fig. 5

sMIC stimulation impairs the expression of genes related to NK cell homeostatic maintenance. Negatively selected mouse splenic NK cells were stimulated with recombinant MICB in the absence or presence of sMIC-clearing mAb B10G5 for 6 h. Total RNA was isolated for RNAseq analyses. R1 and R2 are replicates with two different forms of recombinant sMICB, sMICB-His tagged and sMIC-Fc tagged respectively. a Gene related to survival pathway that are significantly modulated in NK cells by sMIC and rescued with the sMIC-clearing mAb B10G5. b Gene related to proliferation pathway that is significantly modulated in NK cells by sMIC and rescued with the sMIC-clearing mAb B10G5. c Modification of genes in IL-2 signaling pathways by sMIC. d Validation of changes in the expression of representative genes as shown in a–c by qRT-PCR

Back to article page

Journal of Hematology & Oncology

ISSN: 1756-8722

Contact us