Fig. 6
Combined therapy with B10G5 and anti-PDL1 significantly decreases established B16-sMIC+ metastases. a Depiction of treatment scheme. B6/MICB mice received i.v. injection of 4 × 105 B16F10-sMICB cells. At day 10, i.p. treatment was initiated with (1) control mIgG, (2) B10G5, (3) anti-PDL1, and (4) a combination of B10G5 and anti-PDL1. Antibodies were given by i.p. injection every 3 days in 200 μl sterile PBS. b Representative H&E sections of lungs of mice with metastatic B16-sMICB tumors. c Quantitation of micrometastasis in one lung section of all lobes. e, f Enhanced NK cell numbers, CD25 expression, and IFNγ production in draining LNs (two inguinal) of animals receiving combined treatment. Data obtained at day 21 of the experiment. *p < 0.05 as compared to the control group. Arrows show micrometastasis. **p < 0.05, combination therapy as compared to monotherapy