Fig. 3

Deletion of YAP by CRISPR/Cas9 suppressed cell growth both in vitro and in vivo. a Western blot analysis confirmed CRISPR/Cas9-mediated YAP deletion in LY1 and LY3 cells. b Knocking out YAP induced cell cycle arrest in LY1 and LY3 cells, which were arrested in the G0/G1 phase. **p < 0.01. c Representative results for the cell cycle distribution analysis are shown. d LY1 and LY3 cells transfected with a control (sgCon) or sgYAP were treated with doxorubicin at the indicated concentrations for 24 h before being subjected to CCK-8 assay. *p < 0.05, **p < 0.01, ***p < 0.001. e, f Flow cytometry was used to analyze of cell apoptosis in LY1 and LY3 cells transfected with sgCon or sgYAP and treated with doxorubicin. *p < 0.05, **p < 0.01. g A xenograft DLBCL mouse model was established using LY1 cells with YAP knocked out. ***p < 0.001. h The expression levels of Ki67 and c-myc in xenograft tumors were determined. Bar = 20 μm. i, j The expression and location of differentially expressed (DE) mRNAs detected by RNA-seq in DLBCL cells with YAP knocked out were determined. k KEGG pathway analysis of DE molecules was performed