Fig. 4

IGF-1R inhibitors exert cytotoxic effects on DLBCL cells. a LY1 and LY8 cells were treated with IGF-1R inhibitor AG1024 or PPP at the indicated concentrations for 24 h, and cell proliferation was determined by CCK-8 assay (mean ± SD, n = 6). b, c After treatment with an IGF-1R inhibitor (AG1024 or PPP) at the indicated concentrations for 24 h, cellular apoptosis was detected by flow cytometry. Representative results are shown. The results of the quantitative analysis are shown as the mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. d. Western blot analysis of DLBCL cell lines, treated with a single dose of 15 μM AG1024 for 24 h, was performed to determine the expression of the indicated proteins. e Scatter plots show the positive correlation between YAP and IGF1-R mRNA expression in the TCGA cohort