Fig. 5

Regulation of Hippo-YAP signaling by IGF-1R inhibitors. a, b After treatment with the indicated concentrations of AG1024 or PPP for 24 h, LY1 cells were immunoblotted to determine the protein expression of YAP, p-IGF-1R, and t-IGF-1R. c Knockdown of endogenous IGF-1R reduced YAP protein expression in LY1 and LY8 cells. d LY1, LY8, and LY3 cells were treated with PPP (2 μM) for 24 h, and cytoplasmic and nuclear proteins were separated and extracted. YAP expression was detected by western blot analysis. e, f LY1 and LY8 cells were treated with PPP (2 μM) for 24 h, and immunofluorescence staining was performed to evaluate the endogenous expression and subcellular localization of YAP. Bar = 20 μm