Fig. 6

Administration of IGF-1 induced YAP expression in DLBCL cells. a LY1 cells were serum starved for 24 h and treated with IGF-1 (0 to 50 ng/ml) for 30 min. Western blotting was performed to determine the expression of p-IGF-1R and t-IGF-1R. b YAP expression was also assessed in LY1 cells treated with IGF-1(50 ng/ml). c–f IGF-1 rescued the expression levels of YAP which were decreased by the IGF-1R inhibitors AG1024 (15 μM) and PPP (0.5 μM), respectively. Quantitative data for the relative YAP protein levels are reported as the mean ± SD for triplicate experiments. *p < 0.05. g, h A mouse xenograft model was established with LY1 cells and treated with AG1024. Tumor volume was measured. ****p < 0.0001. i The Ki67 expression level was detected in the xenograft model. Bar = 20 μm. j The graphical representation summarizes the regulation of the Hippo-YAP pathway by IGF-1R