Fig. 1

hETV4 transgene is expressed in prostate of two transgenic mice lines with prostate-specific ETV4 expression. a Diagram of the pPB-ETV4 construct used to generate transgenic mice. It contains part of the rat probasin PB promoter (PB), the TMPRSS2-ETV4 fusion cDNA, and the SV40 polyadenylation sequence. b Western blot analysis of Hek 293 cells transiently transfected with an empty vector (CTL), with the expression vectors containing either the full-length ETV4 (ETV4) or the TMPRSS2-ETV4 fusion cDNA (TMPRSS2-ETV4) under the control of the ubiquitous EF1a promoter. Beta actin was used as loading control. c Relative expression levels of ETV4 mRNA measured by qRT-PCR in the prostate lobes (VP, DLP, AP) of ETV4 transgenic mice (lines ETV4 A and ETV4 B) normalized to wild-type mice (WT). The data represent mean and standard error of triplicate analysis in the indicated number of mice (n). VP: ventral prostate; DLP: dorso-lateral prostate; AP: anterior prostate. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. d Representative microphotographs of ETV4 protein expression by immunohistochemistry analysis (magnification × 200) in the prostate of WT mice (left panel) and ETV4 mice (right panel)