Fig. 4

ETV4 regulate p21 (CIP1/WAF1) and p27 (KIP1) expression in human prostate cell lines. a Relative expression levels of CDKN1A, CDKN1B, and ETV4 mRNA measured by qRT-PCR. PC3 cells were transduced with either an anti-ETV4 shRNA or an irrelevant shRNA. RWPE cells were transfected with either an ETV4-expressing vector or an empty vector. The expression changes are indicated as the ratio between the levels of a given transcript in the experiment and the relevant control. Average and standard error values are shown. At least 4 independent experiments have been performed. *P ≤ 0.05, ***P ≤ 0.001. b Representative western blots of p21 and p27 proteins in PC3 cells transduced with either an irrelevant shRNA (CTL) or 2 anti-ETV4 shRNA (shETV4a and shETV4b), and in RWPE cells transfected with either an empty vector (CTL) or an ETV4-expressing vector (+ETV4). Beta-actin is used as loading control. c Bar diagram of p21 and p27 protein levels measured by western blot analysis in (i) PC3 cells transduced with either a specific shRNA (shETV4) or an irrelevant shRNA (CTL), and in (ii) RWPE cells transfected with either an empty vector (CTL) or an ETV4-expressing vector (+ETV4). Average and standard error values of the relative quantification are shown. At least 4 independent experiments have been performed. *P ≤ 0.05