Fig. 2
From: Advances in the assessment of minimal residual disease in mantle cell lymphoma
PCR-based MRD detection methods. qPCR is the gold standard method that uses IgH rearrangements as clonal markers. Nested PCR uses double amplification and considered to be more qualitative. ddPCR is an absolute quantification method that overcomes many of qPCR’s limitations. In this hypothetical situation where each MCL patient undergoes MRD assessment via different PCR methods, nested PCR is not able to meet the quantitative standards and thus, is less sensitive compared with the qPCR and ddPCR