Fig. 3

HSC does not express CSF1R. a Quantitative real-time PCR data demonstrating Csf1r mRNA expression in sorted HSC, MPP, HPC and committed progenitors as well as positive control samples of sorted monocytes, total BM and day 7 (D7) BMM. b Representative flow cytometry histograms of C57BL/6 mouse BM expression of CD115 (CSF1R) in (i) HSC, (ii) MPP, (iii) HPC and (iv) committed progenitors. Population gating strategies are exemplified in Additional file 1: Figs. S5 and S7. The histograms show antibody staining (blue lines) compared to appropriate isotype staining (red lines). c BM isolated from MacGreen mice was assessed by imaging flow cytometry with dot plots showing BM HSPC population gating strategy and subsequently CD115 and MacGreen GFP transgene expression in gated HSC, MPP, HPC and committed progenitors (CP). d Representative image panels showing individual bright field (BF) and specific antibody/transgene fluorophore images for representative cell events selected from either GFP-negative or GFP-positive gates in (c)