Fig. 4
DMOCPTL induced apoptosis and ferroptosis of TNBC cells. a The representative images of cell apoptosis in MDA-MB-231 cells for 48 h at different concentrations of 0.2 μM, 0.5 μM and 1 μM, respectively. b The statistical results of cell apoptosis assays. c Western blot analysis of caspase 9, cleaved caspase 9, caspase 3, cleaved caspase 3, PARP, and cleaved PARP after treatment of DMOCPTL for 48 h in MDA-MB-231 cells at different concentrations of 0.05 μM, 0.1 μM and 0.2 μM, respectively. d Western blot analysis of apoptosis related proteins of mitochondrial pathway after the treatment of DMOCPTL for 48 h in MDA-MB-231 at different concentrations of 0.05 μM, 0.1 μM and 0.2 μM, respectively. e The representative images and statistical results of ROS after the treatment of DMOCPTL with a dose-dependent manner for 2 h in MDA-MB-231 cells. f The representative images and statistical results of ROS after the treatment of DMOCPTL with a dose-dependent manner for 2 h in SUM159 cells. g The representative images and statistical results of ROS after the treatment of DMOCPTL with a time-dependent manner in MDA-MB-231 cells. h The representative images and statistical results of ROS after the treatment of DMOCPTL with a time-dependent manner in SUM159 cells. i The statistical results of lipid ROS after the treatment of DMOCPTL with a dose-dependent manner for 2 h in MDA-MB-231 and SUM159 cells. j The statistical results of lipid ROS after the treatment of DMOCPTL with a time-dependent manner for 2 h in MDA-MB-231 and SUM159 cells. k The representative images and statistical results of intracellular Fe2+ by PGSK assay after the treatment of DMOCPTL for 48 h in MDA-MB-231 cell. l The fluorescence intensity of PGSK after the treatment of DMOCPTL for 48 h in MDA-MB-231 cells by immunofluorescence assay. Analysis results represented mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001