Fig. 4

Functional analysis of B7-H3 CAR-T cells. a Cytokine production in B7-H3 CAR and vehicle T cells. Effector T cells were incubated with target A549 or HCT 116 cells at a ratio of 10:1 for 24 h. The concentrations of cytokines were measured using flow cytometry. b Memory cell phenotypes of B7-H3 CAR-T cells. Effector T cells were incubated with target A549 or HCT 116 cells at a ratio of 10:1 for 24 h. Expression of CD62L and CCR7 on T cells was identified with staining with anti-CD62L and anti-CCR7 antibodies, respectively, and measured using flow cytometry. c Degranulation markers in B7-H3 CAR T cells. Effector T cells were incubated with target A549 or HCT 116 cells at a ratio of 10:1 for 6 h (CD107a) or 24 h (granzyme B and perforin). Expressions of CD107a, intracellular granzyme B and perforin staining on T cells were stained with anti CD107a, granzyme B and perforin antibodies and measured by flow cytometry. Data from 2–3 independent experiments are presented as the mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001. The p values were analyzed using a Student’ t test