Fig. 6
STOML2 is upregulated by Lenvatinib in HCC with HIF-1α dependent. a Western blot analysis of STOML2 and HIF-1α expression in both SMMC-7721 and HCCLM3 cells after exposure to lenvatinib (10 μM) for 24 h and 48 h. b Knockdown of HIF-1α expression in both SMMC-7721 and HCCLM3 cells counteracted the upregulation of STOML2 caused by lenvatinib. c qRT-PCR analysis of STOML2 expression in both SMMC-7721 and HCCLM3 cells after exposure to lenvatinib (10 μM) for 24 h and 48 h. d Endogenous HIF-1α associated with the STOML2 promoter. ChIP qRT-PCR assay was performed with sonicated chromatins immunoprecipitated from HCCLM3 cells by anti-HIF-1α mAb or preimmune IgG (control). e CTRL, WT and Mut reporter were exposed to 20% O2 or 1% O2 for 12 h in the SMMC-7721 cells. f Overexpression of HIF-1α stimulated the STOML2 promoter reporter activity in SMMC-7721 cells (left panel). Knockdown of HIF-1α expression downregulated the STOML2 promoter reporter activity in the HCCLM3 cells (right panel). *P < 0.05; **P < 0.01; ***P < 0.001; ns no significance, Mut mutation, CTRL control