Fig. 1
From: Clonal evolution in liver cancer at single-cell and single-variant resolution
Single-cell analysis revealed a common origin but independent evolution of primary and metastatic liver tumors. a Overview of the single-cell analysis strategy of human liver cancer. b Mutational status of SNV/INDEL sites in single cells from paratumor, primary tumor and PVTT tissues in HCC8. c Clonal evolution in HCC8 with genes mutated at each step shown. Dashed circle: virtual ancestor clone in PVTT. *COSMIC Cancer Gene Census catalogued driver genes. d Statistics of nucleotide substitution types for clone-specific point mutations newly acquired from the most recent ancestor in HCC8 as shown in (c). e Maximum parsimony tree of single cells from HCC8 based on nucleotide sequences at the target sites. Scale bar: nucleotide substitution rate