Fig. 3

NONO-TFE3 inhibits lncRNA TRAF3IP2-AS1 transcription. a ChIP assays showed endogenous NONO-TFE3 binding to the TRAF3IP2-AS1 gene promoter. 786-O cells were transfected with NONO-TFE3-Flag-overexpressing vector for 48 h. The binding of NONO-TFE3 at the TRAF3IP2-AS1 promoter region was detected by a ChIP assay. b Schematic summary of the dCas9-gRNA-guided ChIP (upper); Western blot was performed after dCas9-gRNA-guided ChIP (lower). c–d HEK293T cells were co-transfected with TRAF3IP2-AS1 promoter–luciferase truncations and NONO-TFE3 plasmids, and the luciferase activity was determined using a Dual Luciferase Reporter Assay after 48 h. e Dual luciferase assay of HEK293T cells co-transfected with firefly luciferase constructs containing the wild-type or mutant NONO-TFE3 potential binding sites of TRAF3IP2-AS1 promoter and NONO-TFE3 plasmids were performed. g The protein and mRNA levels of NONO-TFE3 and the TRAF3IP2-AS1 expression levels were detected after transfection with NONO-TFE3 plasmids or shTFE3 for 48 h. h NONO-TFE3 immunohistochemistry was performed in paraffin sections of samples from patients. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001