Fig. 5
Effect of VEN plus 8‐Cl‐Ado combinatorial treatment on growth of AML cells in vitro. Effect of VEN plus 8‐Cl‐Ado combinatorial treatment on the growth of AML cell lines KG-1a and MV4-11 (a) and primary CD34+CD38− AML blasts (b). Cells were treated for 48 h with control vehicle, individual drugs or with the 2-drug combination at constant drug ratios, on the basis of previously established IC50 values, followed by measurement of cell proliferation. Drug synergy was analyzed using the CalcuSyn program. Data are presented as mean ± SE, with quadruplicate determination. Results from one representative experiment of 2 independent experiments are shown. Combination index blots and combination indices (CI) are presented. Drug synergism, addition, and antagonism effects are defined by combination index values of < 1.0, 1.0, and > 1.0, respectively. c Intracellular concentration of 8‐Cl‐ATP in MV4-11 cells (2 × 105 cells/mL) treated with 250 nM 8‐Cl‐Ado, 10 nM VEN, or both, for up to 12 h. Results from one representative experiment (in duplicates) of 2 independent experiments are shown