Fig. 6

LA-ISA immunized spleen lymphocytes from HLA-A2.1 transgenic mice against MDA-MB-231 cell-bearing NOD/SCID mice. a Immunological reconstruction pattern diagram. b Immunological reconstitution in tumour-bearing NOD-SCID mice was constructed using splenic lymphocytes harvested from HLA-A2.1 transgenic mice immunized subcutaneously with YL-ISA, LA-ISA, ISA, or control (1 mg epitope/20 g body weight). Growth curve and weight of transplanted tumour at day 25 after tumour cell inoculation (n = 10). c Representative immunohistochemical staining of CD8a and NK1.1 in subcutaneously transplanted tumour tissues. d Survival (n = 10). AVG, average; LER (%), life extension rate. e Immunological reconstitution in a metastatic NOD/SCID mouse model was constructed using splenocytes harvested from HLA-A2.1 transgenic mice immunized subcutaneously with YL-ISA, LA-ISA, ISA, or control (1 mg epitope/20 g body weight). Representative bioluminescence images and quantitative data of mice and vital organs (n = 3). Representative macroscopic images and quantitative data of pulmonary metastases (n = 6). Lu, lung; Li, liver; H, heart; S, spleen; K, kidney. f H&E staining of pulmonary metastases. Immunohistochemical staining of CD8a and NK1.1 in pulmonary metastases. g Survival (n = 6). AVG, average; LER (%), life extension rate. c, f Scale bars, 50 μm. Control, NOD/SCID mice inoculated with splenocytes from HLA-A2.1 transgenic mice treated with normal saline. ISA, NOD/SCID mice inoculated with splenocytes from HLA-A2.1 transgenic mice treated with ISA. YL-ISA, NOD/SCID mice inoculated with splenocytes from HLA-A2.1 transgenic mice treated with YL-ISA. LA-ISA, NOD/SCID mice inoculated with splenocytes from HLA-A2.1 transgenic mice treated with LA-ISA. b, e P values were obtained with independent-samples t-test; error bars denote standard deviation (SD); d, g P values were calculated by the log-rank test; b, d, e, g Compared with control, ^**P < 0.01, ^***P < 0.001; compared with ISA, ^##P < 0.01, ^###P < 0.01; compared with YL-ISA, ^&P < 0.05, ^&&P < 0.01