Fig. 3

Optimization strategies for CAR-NK cell generation. a Different vectors can be used to transduce NK cell lines and primary NK cells with CAR constructs. Quantification of the vectors used for CAR-NK generation. b Details of the various vectors used for CAR-NK generation. In blue, lentivirus generation, with third-generation viruses considered the safest option with minimal risk of in vivo recombination with other lentiviruses. In purple, the genus of retrovirus (alpha or gamma, or unknown). In green, the electroporated vector. c Various compounds have been identified that can be used to enhance virus-mediated transduction. For studies that employ virus-based strategies for CAR-NK generation, the methods used for enhancing virus transduction were quantified. d Primary cells for generation of CAR-NK cells are derived from different sources. Quantification of the four sources for primary NK cells for CAR-NK generation