Table 6 Approaches for NK cell expansion
From: Chimeric antigen receptor natural killer (CAR-NK) cell design and engineering for cancer therapy
Approach | Advantages | Disadvantages |
|---|---|---|
Cytokine mixture | Feeder free | Relatively long procedure |
Easy | May induce NK cell exhaustion | |
K562 or other feeder cell-based expansion | Rapid expansion | Risks from the feeder cells (contamination, tumorigenesis) |
Elongation of telomeres (only with IL21) | Â | |
Autologous PBMC feeder cells | Safe | Need for autologous cells |
Well-known | Expansion limited compared to other techniques | |
Super agonist-based expansion | Feeder free | Technology is complicated |
In vivo expansion | Â |