Fig. 1

PVRIG is mainly expressed on lymphocytes and associated with poor clinical outcomes in patients with colon adenocarcinoma. a Representative immunohistochemical staining of PVRL2 in the peritumoral and intratumoral tissues of colon adenocarcinoma (COAD) patients. Original magnifications: 20×. Bar = 50 μm. b Representative immunohistochemical staining of PVRIG in the peritumoral and intratumoral tissues of COAD patients. Positive staining is indicated by red arrows. Original magnifications: 20X. Bar = 50 μm. c Correlation between PVRIG and other immune checkpoint receptor (TIGIT, CD96 or PDCD1) based on the gene expression analysis of COAD patients (n = 329 per group, TCGA database). d Kaplan–Meier survival of COAD patients (n = 88 per group, cutoff: 20–20, TCGA database) based on the level of PVRIG mRNA expression (PVRIG low, blue; PVRIG high, red). Data were analyzed for significance by the log-rank test