Fig. 1

Selective gamma-secretase inhibitor MRK-560 inhibits cell growth in NOTCH1-dependent T-ALL cell lines. a Western blot analysis of PTEN protein levels in 8 T-ALL cell lines. Beta-actin was used as loading control. b Western blot analysis of cleaved NOTCH1 after treatment with DMSO (D), MRK-560 1 µM (M) or PF-03084014 1 µM (P) for 24 h. Beta-actin was used as loading control for all conditions; separate boxes represent separate blots. c Growth curves for DND-41, RPMI-8402, ALL-SIL, MOLT-4, HPB-ALL, HSB-2, JURKAT and LOUCY T-ALL cell lines. Growth medium was supplemented with DMSO, MRK-560 0.1 µM or MRK-560 1 µM. Data are shown as mean of biological triplicates. Standard deviation is depicted (only visible when larger than used symbols)