Fig. 1

LCAT3 is upregulated in lung tumor tissues and associated with poor prognosis of LUAD. A Boxplot showing the relative expression of LCAT3 in lung tumors (n = 485) and adjacent normal (n = 56) tissues in a TCGA LUAD cohort. LCAT3 expression was quantified by FPKM in the RNA-seq data. B LCAT3 expression validated by qRT-PCR in an independent cohort of 13 paired samples of LUAD tissues and adjacent normal tissues. LCAT3 expression was normalized to the expression of β-actin. C, D Kaplan–Meier curves of overall survival (C) and disease-free survival (D) of lung cancer patients with high versus low expressions of LCAT3. E, F The protein coding potential of LCAT3 evaluated by the Coding Potential Assessment Tool (CPAT) and Coding Potential Calculator 2 (CPC2). Two verified lncRNAs, XIST and HORAIR, served as controls for the prediction. G, H Subcellular localization of LCAT3. Real-time PCR analysis confirmed the nuclear and cytoplasmic fraction of LCAT3 transcript in Calu1 and Hop62 cells; U6 and GAPDH served as positive controls for the nuclear and cytoplasmic fractions, respectively